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Applied Molecular Biology

Course Description: This course focuses on the practical applications of molecular biology principles and techniques in the fields of biotechnology, veterinary medicine, agriculture, forensics, and biomedical research. Students gain an understanding of advanced molecular methods used to analyze, manipulate, and modify genetic material. The course covers topics such as recombinant DNA technology, polymerase chain reaction (PCR), DNA sequencing, gene cloning, gene expression analysis, molecular diagnostics, gene editing technologies, and bioinformatics tools. It also emphasizes the application of molecular biology techniques to solve real-world biological and medical problems, analyze experimental data, and evaluate scientific advancements in the field of molecular biotechnology. The course includes laboratory activities and case studies to develop students' practical skills and critical thinking.
Credit hours: 3
Objectives of the course :

By the end of this course, the student is expected to be able to:

 

1. Explanation of the principles and applications of modern techniques in molecular biology in the fields of biotechnology, veterinary medicine, agriculture, and scientific research.
2. Application of molecular biology techniques to analyze genetic material and study various biological problems.
3. Explanation of the principles and applications of recombinant DNA technology, gene cloning, and genetic engineering.
4. The use of molecular techniques such as polymerase chain reaction (PCR), DNA sequencing, electrophoresis, and gene expression analysis.
5. Analysis and interpretation of data generated from molecular experiments and studies.
6. Explain the role of molecular diagnostics in detecting diseases, monitoring them, and contributing to their treatment.
7. Discussion of gene editing technology applications and their associated ethical aspects.
8. Employing bioinformatics tools in the analysis of genomic and molecular data.
9. Evaluating developments and emerging technologies in the field of applied molecular biology.
10. Developing critical thinking, problem-solving, and laboratory skills necessary for modern molecular applications.

Course outputs :

By the end of this course, the student is expected to be able to:

 

I: Knowledge and understanding:

1. It explains the fundamental principles and modern applications of molecular biology in the fields of veterinary medicine, biotechnology, agriculture, and forensic sciences.
2. It describes the mechanisms and techniques for analyzing and modifying genetic material used in modern molecular applications.
3. It explains the principles of molecular diagnostics, genetic engineering, and gene editing techniques.
4. Explains the role of bioinformatics in the analysis of molecular and genomic data.

II: Skills:

1. Applies basic and advanced molecular techniques such as PCR, electrophoresis, DNA sequencing, and gene expression analysis.
Analyzes and interprets the data and results generated from molecular experiments and applications.
3. Uses appropriate bioinformatics software and databases to analyze genetic and genomic information.
4. Employs scientific thinking methods to solve biological and medical problems using molecular techniques.
5. Evaluates recent studies and research in the field of applied molecular biology using scientific evidence.

Third: Values, Independence, and Responsibility:

1. He adheres to the ethics of scientific research and biosafety standards when handling samples and biological materials.
2. Works effectively within a team in executing activities, experiments, and applied projects.
3. He is responsible for self-learning and following up on modern scientific and technical developments in the field of molecular biology.
4. Demonstrates scientific communication skills in presenting and discussing results and molecular applications.
5. Makes scientific and professional decisions based on evidence and ethical considerations in molecular applications.

Additional information:

* Credit hours: 2 credit hours.
* Course level: Master's degree.
Language of Instruction: English.

 

Course Main Topics:

1. Introduction to Applied Molecular Biology.
2. Recombinant DNA Technology.
3. Gene cloning and genetic vector systems.
4. Polymerase Chain Reaction (PCR) and Quantitative Polymerase Chain Reaction (Real-Time PCR).
5. DNA Sequencing techniques.
6. Gene expression analysis.
7. Molecular diagnosis and biomarker detection.
8. Genome editing technologies (CRISPR-Cas systems).
9. Molecular applications in veterinary medicine and healthcare.
10. Molecular applications in agriculture and food biotechnology.
11. Forensic Molecular Biology.
12. Bioinformatics and genomic data analysis.
13. Modern trends in molecular biotechnology.
14. Ethical, Legal, and Social Issues in Molecular Biology.

Teaching Strategies:

Interactive lectures.
* Laboratory experiments.
Case studies.
Problem-based learning.
* Discussion of research papers and scientific articles.
Student presentations.
Group projects.

Evaluation Methods

* Quizzes and assignments.
* Laboratory reports.
* Practical exams.
Midterm exam.
* Research project or case study presentation.
Final test.

Suggested References:

* Brown, T. A. Gene Cloning and DNA Analysis: An Introduction.
Primrose, S. B., & Twyman, R. M. Principles of Gene Manipulation and Genomics.
Glick, B. R., Pasternak, J. J., & Patten, C. L. (2010). *Molecular Biotechnology: Principles and Applications of Recombinant DNA*.
* Watson, J. D. et al. Molecular Biology of the Gene.
* Lodish, H. et al. Molecular Cell Biology.

Teaching hours:

Lectures: 1 hour per week.
Lab: 1 hour per week.

Additional notes:

This course focuses on the practical application of molecular biology techniques in scientific research, molecular diagnostics, biotechnology, and healthcare. Students gain hands-on experience using modern molecular tools and techniques, and develop the analytical, technical, and problem-solving skills necessary for careers in biotechnology, biomedical sciences, molecular diagnostics, and advanced scientific research.

  • Last update : 2026-09-06

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