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Laboratory technique in maxillofacial pathology

Course Description: This elective course will focus on basic and advanced laboratory techniques in the field of oral and maxillofacial diseases. Techniques such as (laser, and radionuclide implantation) will encourage students, dentists, and oral surgeons to conduct human research, as well as experimental animal models, and utilize computer programs for the analysis of microscopic results in diagnosis and research. Additionally, this course will highlight protection methods against infection, and the physical, chemical, and biological risks of radiation in dental clinics and laboratories.

Credits: 2

Prerequisites: DENT 114

Objectives of the course :

By the end of this course, the student should be able to:
• Directs himself with laboratory facilities including biological equipment and tools, microtomes, histochemical processors, regular and diamond knives, fixatives and chemicals, paraffin wax, regular and special stains, glass slides, microscopes, incubators, ovens, etc.
Understanding biological changes at the cellular and tissue level from the moment a surgical biopsy/smear is obtained from the patient.
• Preservation of biopsies / oral cell smears, especially in the field of chemicals.
• Tissue recognition, including processing paraffin-embedded specimens, sectioning, staining, and microscopic examination.
* Be familiar with the following technologies:
• Standard preparation of soft tissues, teeth, and bone, and smears for light microscopy
Chemical tissue preparation for soft tissues and bones, cryostat smears, and paraffin sections.
• Preparation of immunocytochemical tissue sections using specific markers (tumor markers) for both electron microscopy (transmission and scanning) and light microscopy.
Preparation of tissue culture for soft tissues and bones, and smears for light or electron microscopy.

Course outputs :

• Radiography using radioactive elements is a technique for studying and monitoring radioactive isotopes in soft and hard tissues for diagnostic and research purposes.
• Preparation of soft/hard tissue for laser and implant application in dentistry.
• Create a pathology report.
Linking clinical and microscopic information to establish an accurate diagnosis.
• Knowledge of interpreting radiological images, particularly concerning complex oral diseases.
Discuss the clinical/pathological relationship in oral disease cases.
The precise management of oral diseases, out of fear of infection and contamination from hazardous materials in clinics.
Exercise great care and take precautions when processing laboratory samples from individuals infected with highly contagious (viral) diseases or contaminated with (radioactive isotopes).

Additional information:

Last updated: 2026-04-08